期刊
ARCHIVES OF MICROBIOLOGY
卷 190, 期 5, 页码 595-603出版社
SPRINGER
DOI: 10.1007/s00203-008-0407-1
关键词
iron transportation; TonB system; Vibrio alginolyticus; virulence
类别
资金
- Chinese National Natural Science Foundation [U0633004]
- Shanghai Leading Academic Discipline Project [B505]
In Gram-negative bacteria, the TonB/ExbB/ExbD complex is required to energize the specific high-affinity receptors mediating iron uptake processes. In fish pathogen Vibrio alginolyticus MVP01, which exhibited capacities to scavenge iron sources with various specific iron uptake systems, we identified and characterized two sets of TonB systems. In V. alginolyticus, TonB1 and TonB2 systems were arrayed as TonB1-ExbB1-ExbD1 and ExbB2-ExbD2-TonB2, respectively. The transcription of the TonB systems was regulated by the iron concentration in culture medium. While TonB1 system specifically contributed to the hemin and hemoglobin utilization in V. alginolyticus MVP01, both of the TonB systems showed abilities to support the utilization of iron sources from ferrichrome and vibrioferrin, the endogenous siderophore of V. alginolyticus. In addition, when mutants of the TonB systems were inoculated intraperitoneally into the fish Brachydanio rerio, marked attenuation in virulence of V. alginolyticus was observed, indicating that both of the TonB systems are essential for the virulence of V. alginolyticus.
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