期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 69, 期 10, 页码 6327-6333出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.69.10.6327-6333.2003
关键词
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A multiplex real-time PCR method to simultaneously detect the stx1 and stx2 genes of Shiga toxin-producing Escherichia coli and a unique conserved single-nucleotide polymorphism in the E. coli O157:H7/H- uidA gene has been developed. There is more than 98.6% sensitivity and 100% specificity for all three gene targets based on a panel of 138 isolates. The PCR efficiencies were greater than or equal to1.89, and as few as 6 CFU/reaction could be detected.
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