期刊
JOURNAL OF STRUCTURAL BIOLOGY
卷 144, 期 1-2, 页码 114-122出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2003.09.028
关键词
bilateral filter; denoising; electron cryomicroscopy; image processing; 3D reconstruction
资金
- NCRR NIH HHS [P41RR02250] Funding Source: Medline
- NIAID NIH HHS [R21AI053737] Funding Source: Medline
- NLM NIH HHS [T15LM07093, T15 LM007093] Funding Source: Medline
Due to the sensitivity of biological sample to the radiation damage, the low dose imaging conditions used for electron microscopy result in extremely noisy images. The processes of digitization, image alignment, and 3D reconstruction also introduce additional sources of noise in the final 3D structure. In this paper, we investigate the effectiveness of a bilateral denoising filter in various biological electron microscopy applications. In contrast to the conventional low pass filters, which inevitably smooth out both noise and structural features simultaneously, we found that bilateral filter holds a distinct advantage in being capable of effectively suppressing noise without blurring the high resolution details. In as much, we have applied this technique to individual micrographs, entire 3D reconstructions, segmented proteins, and tomographic reconstructions. (C) 2003 Elsevier Inc. All rights reserved.
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