期刊
JOURNAL OF GENERAL PHYSIOLOGY
卷 122, 期 4, 页码 407-417出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1085/jgp.200308843
关键词
ryanodine receptor; Ca2+ release; sarcoplasmic reticulum
类别
资金
- ARRA NIH HHS [RA32808] Funding Source: Medline
- NHLBI NIH HHS [R01 HL064210, HL64210, HL57832, R01 HL057832] Funding Source: Medline
Ryanodine receptor (RyR) channels from mammalian cardiac and amphibian skeletal muscle were incorporated into planar lipid bilayers. Unitary Ca2+ currents in the SR lumen-to-cytosol direction were recorded at 0 mV in the presence of caffeine (to minimize gating fluctuations). Currents measured with 20 mM lumenal Ca2+ as exclusive charge carrier were 4.00 and 4.07 pA, respectively, and not significantly different. Currents recorded at 1-30 mM lumenal Ca2+ concentrations were attenuated by physiological [K+] (150 mM) and [Mg2+] (I mM), in the same proportion (similar to55%) in mammalian and amphibian channels. Two amplitudes, differing by similar to35%, were found in amphibian channel studies, probably corresponding to alpha and beta RyR isoforms. In physiological [Mg2+], [K+], and lumenal [Ca2+] (I MM), the Ca2+ current was just less than 0.5 pA. Comparison of this value with the Ca2+ flux underlying Ca2+ Sparks suggests that sparks in mammalian cardiac and amphibian skeletal muscles are generated by opening of multiple RyR channels. Further, symmetric high concentrations of Mg2+ substantially, reduced the current carried by 10 mM Ca2+ (similar to40% at 10 MM Mg2+), suggesting that high Mg2+ may make sparks smaller by both inhibiting RyR gating and reducing unitary current.
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