期刊
EXPERIMENTAL EYE RESEARCH
卷 77, 期 4, 页码 485-495出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/S0014-4835(03)00154-4
关键词
corneal endothelial cells; L-type Ca2+ channels; growth factor receptor; tyrosine kinase; intracellular Ca2+; capacitative Ca2+ entry
This study was undertaken to investigate electrophysiological properties of immortalized SV40-transfected human corneal endothelial cells (HCEC-SV40) combined with the analysis of intracellular Ca2+ responses mediated by ligands for receptor tyrosine kinases (RTK). In addition, the effects of several tyrosine kinase inhibitors were tested on Ca2+ inflow mediated by induction of capacitative calcium entry (CCE). Patch-clamp techniques and measurements of the intracellular free Ca2+ ([Ca2+](j)) by fura-2 were performed using HCEC-SV40. Stimulation of fibroblast growth factor receptors (FGFR) (e.g. by basic-FGF) (10 ng ml(-1)) elicited activation of Ca2+ permeable channels and a subsequent increase of cytosolic free Ca2+ in HCEC-SV40. This effect could be disrupted by the L-type Ca2+ channel blocker nifedipine (5 muM). In addition, nifedipine significantly reduced the magnitude of CCE. Inhibition of protein tyrosine kinases (PTKs) by genistein, lavendustin A, or tyrphostin 51 (all 5 p,m) also led to a reduction of CCE in HCEC-SV40. This study demonstrates for the first time that L-type Ca2+ channel activity in HCEC-SV40 is linked to the activity of FGF receptor tyrosine kinases. These data regarding Ca2+ inflow through Ca2+ channels could be useful for investigation of culture and vitality conditions of HCEC. (C) 2003 Elsevier Ltd. All rights reserved.
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