期刊
BRAIN BEHAVIOR AND IMMUNITY
卷 17, 期 5, 页码 393-402出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S0889-1591(03)00028-X
关键词
T and B lymphocytes; neuro-immune interactions; FMN; acquired immunity; reconstitution; neuronal survival
资金
- NINDS NIH HHS [NS40433] Funding Source: Medline
The capacity of facial motor neurons (FMN) to survive injury and successfully regenerate is substantially compromised in immunodeficient mice, which lack T and B lymphocytes (Serpe et al., 1999). The goal of the present study was to determine which T cell subset (CD4+ and/or CD8+), and whether the B lymphocyte, is involved in FMN survival after nerve injury. All mice were subjected to a right facial nerve axotomy, with the left (uncut) side serving as an internal control. FMN survival, of the right (cut) side, was measured 4 weeks post-operative, and expressed as a percentage of the left (uncut) control side. FMN survival in wild-type mice was 86% +/- 1.5. In contrast, FMN survival in CD4 KO mice was 60% +/- 2.0. Reconstitution of either CD4 KO mice, or recombinase activating gene-2 knockout (RAG-2 KO) mice (which lack functional T and B cells) with CD4+ T cells alone restored FMN survival to wild-type levels (85% +/- 1.2 and 84% +/- 2.5, respectively). There was no difference in FMN survival between wild-type, CD8 KO and MmuMT (B cell deficient) mice. Reconstitution of RAG-2 KO mice with CD8+ T cells alone, or B cells alone, failed to restore FMN survival levels (65% +/- 1.5 and 63% +/- 1.0, respectively). It is concluded that, of the population of FMN that do not survive injury, CD4+ T lymphocytes, but not CD8+ T lymphocytes or B cells, mediate FMN survival after peripheral nerve injury. (C) 2003 Elsevier Science (USA). All rights reserved.
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