期刊
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
卷 285, 期 4, 页码 H1626-H1631出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpheart.00332.2003
关键词
cardiac; heart; lactate; metabolism
资金
- NHLBI NIH HHS [HL-64848] Funding Source: Medline
This study tested the hypothesis that an acute infusion of beta-hydroxybutyrate inhibits myocardial fatty acid uptake and oxidation in vivo. Anesthetized pigs were untreated (n = 6) or treated with an intravenous infusion of fat emulsion ( n = 7) to elevate plasma free fatty acid levels. A third group received fat emulsion plus an intravenous infusion of beta-hydroxybutyrate (25 mumol . kg(-1) . min(-1); n = 7) for 60 min. All animals received a continuous infusion of [H-3] palmitate, and myocardial fatty acid oxidation was measured from the cardiac production of (H2O)-H-3. Plasma free fatty acid concentrations were elevated in the fat emulsion group (0.77 +/- 0.11 mM) compared with the untreated group (0.15 +/- 0.03 mM), which resulted in greater myocardial free fatty acid oxidation. In contrast, the group receiving beta-hydroxybutyrate in addition to fat emulsion had elevated beta-hydroxybutyrate concentration (0.87 +/- 0.11 vs. 0.04 +/- 0.01 mM), but suppressed fatty acid oxidation (0.053 +/- 0.013 mumol . g(-1) . min(-1)) (P < 0.05) compared with the fat emulsion group (0.116 +/- 0.029 μmol • g(-1) • min(-1)). There were no differences among the three groups in the tissue content for malonyl-CoA, acetyl-CoA, or free CoA or the activity of acetyl-CoA carboxylase; thus the inhibition of fatty acid oxidation by elevated β-hydroxybutyrate did not appear to be due to malonyl- CoA inhibition of carnitine palmitoyl transferase-I or to an increase in the acetyl-CoA-to-free CoA ratio. In conclusion, fatty acid uptake and oxidation is blocked by an infusion of β-hydroxybutyrate; this effect was not due to elevated myocardial malonyl- CoA content.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据