期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 67, 期 10, 页码 2075-2082出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.67.2075
关键词
Coriolus hirsutus; basidiomycete; transformation; ornithine carbamoyltransferase
An efficient transformation system for the basidiomycete Coriolus hirsutus was developed. A double-auxotrophic mutant of C. hirsutus, deficient both in ornithine carbamoyltransferase (OCTase) and 3-isopropylmalate dehydrogenase (3-IPM dehydrogenase), was transformed to Arg(+) with each allelic type of the C. hirsutus genomic OCTase gene (arg1) newly cloned. The transformation frequency of 10(3)-10(4) transformants per mug DNA per 10(6)-10(7) oidial protoplasts was reached. Southern blots showed that the transforming DNA was integrated into chromosomal DNA with multi-copies. The Arg(+) phenotype of the transformants was stably inherited through mitosis.
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