期刊
INFECTION AND IMMUNITY
卷 71, 期 10, 页码 5871-5880出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/IAI.71.10.5871-5880.2003
关键词
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A unique feature of Citrobacter koseri is the extremely high propensity to initiate brain abscesses during neonatal meningitis. Previous clinical reports and studies on infant rats have documented many Citrobacter-filled macrophages within the ventricles and brain abscesses. It has been hypothesized that intracellular survival and replication within macrophages may be a mechanism by which C koseri subverts the host response and elicits chronic infection, resulting in brain abscess formation. In this study, we showed that C koseri causes meningitis and brain abscesses in the neonatal rat model, and we utilized histology and magnetic resonance imaging technology to visualize brain abscess formation. Histology and electron microscopy (EM) revealed that macrophages (and not fibroblasts, astrocytes, oligodendrocytes, or neurons) were the primary target for long-term C koseri infection. To better understand C koseti pathogenesis, we have characterized the interactions of C koseri with human macrophages. We found that C koseti survives and replicates within macrophages in vitro and that uptake of C koseri increases in the presence of human pooled serum in a dose-dependent manner. EM studies lend support to the hypothesis that C koseri uses morphologically different methods of uptake to enter macrophages. FcgammaRI blocking experiments show that this receptor primarily facilitates the entry of opsonized C koseri into macrophages. Further, confocall fluorescence microscopy demonstrates that C koseri survives phagolysosomal fusion and that more than 90% of intracellular C koseri organisms are colocalized within phagolysosomes. The ability of C koseri to survive phagolysosome fusion and replicate within macrophages may contribute to the establishment of chronic central nervous system infection including brain abscesses.
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