Two dimensional auto-organized nanostructure formation of hyaluronate on bovine serum albumin monolayer and its surface properties

The layer-by-layer interaction between sodium hyaluronate (NaHA) and bovine serum albumin (BSA) were studied by a quartz crystal microbalance QCM) method. Their surface structures, adhesive force F-ad and frictional behavior were investigated using an atomic force microscope (AFM). The adsorptions of NaHA on the BSA monolayer were found to be the Langmuir type. The surface structures of NaHA adsorbed on the BSA monolayer were found to form hexagonal-like networks and the mesh size decreased with increasing molecular weight M, The Fad between the AFM tip and the surfaces of the saturated NaHA layers increased with increasing value of M, However, the frictional coefficient of saturated NaHA layer was found to decrease from 0.933 to 0.191.