期刊
NEUROSCIENCE LETTERS
卷 350, 期 3, 页码 173-177出版社
ELSEVIER SCI IRELAND LTD
DOI: 10.1016/S0304-3940(03)00881-4
关键词
peroxynitrite; apoptosis; vascular smooth muscle cells; canine cerebral arteries; [Ca2+]
资金
- NIAAA NIH HHS [AA-08674] Funding Source: Medline
Considerable evidence is accumulating to suggest that in vivo formation of free radicals in the brain, such as peroxynitrite (ONOO-), and programmed cell death (i.e. apoptosis) play important roles in neurodegeneration and stroke. However, it is not known whether ONOO- can induce apoptosis in cerebral vascular smooth muscle cells (CVSMCs). The present study was designed to determine whether or not canine CVSMCs undergo apoptosis following treatment with ONOO-. Direct exposure of canine CVSMCs to ONOO- induced apoptosis in a concentration-dependent manner, as confirmed by means of fluorescence staining, TdT-mediated dUTP nick-end labeling and comet assays. Peroxynitrite treatment resulted in an elevation of [Ca2+](i) in the CVSMCs. Peroxynitrite-induced apoptosis may thus be brought about by activation of Ca2+-dependent endonucleases. Although the precise mechanisms by which peroxynitrite induces apoptosis need to be further investigated, the present findings could be used to suggest that ONOO- formation in the brain may play important roles in neurodegenerative processes and strokes via detrimental actions on cerebral microvessels and blood flow. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
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