期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 310, 期 1, 页码 196-201出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2003.09.011
关键词
VR1; vanilloid receptor; overexpression; fusion proteins; protein purification; fura2
The vanilloid receptor type 1 (VR1) is a novel membrane receptor activated by heat or chemical ligands conveying information about chemosensitive and thermosensitive pain. We have overexpressed and purified wild type VR1 (wtVR1) as well as several mutant forms using the yeast strain Saccharomyces cerevisiae with the goal of obtaining sufficient protein for structural studies. To facilitate the rapid assaying of protein production and purification we used PCR to construct mutant VR1-green fluorescent protein fusion genes. All recombinant inserts were engineered with 12 HIS tags on the C-terminus for metal affinity column purification. The yield of purified protein from 16 L fermentation was about 1 mg following a single-step purification procedure. By taking advantage of the calcium permeability of VR1 we measured changes in [Ca2+](i) in capsaicin-stimulated fura-2 loaded yeast cells expressing VR1. (C) 2003 Elsevier Inc. All rights reserved.
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