A novel hierarchical nanozeolite composite as sorbent for protein separation in immobilized metal-ion affinity chromatography

A new hierarchical porous zeolite-diatomite composite (see Figure inset) of excellent mechanical and chemical stability, after immobilization of Co2+ by an ion-exchange process, was utilized as a packing sorbent for the separation of peptides and proteins containing histidine clusters. The Figure shows the chromatographic profile for two peptides separated-using immobilized metal-ion affinity chromatography.