期刊
TALANTA
卷 61, 期 2, 页码 173-179出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0039-9140(03)00247-9
关键词
flow injection; on-line solid-phase reagent; iodine; ascorbic acid determination
A flow injection analysis (FIA)-background correction method comprising two solid-phase reactors and spectrophotometry for determination of ascorbic acid (AsA) is proposed. A polyethylene mini-column filled with solid iodine (30% m/m suspended on silica gel beads), reactor 1, and other column filled only with silica gel, reactor 2, which are then incorporated in a flow system so that solid iodine reagent in reactor I is affected as the sample passes through the column. The sample blank is produced by the oxidation of the AsA by iodine to form dehydroascorbic acid, insensitive to ultraviolet at 267 nm. AsA in samples is determined after injected in reactor 2; the difference in two analytical signal observed is related to amount of AsA. The linear range of the system is up to 50 mug ml(-1) with a detection limit of 0.08 mug ml(-1) R.S.D. of better than 1.0% and sampling frequency of 110 sample h(-1). The method is successfully applied to the determination of AsA in pharmaceuticals and foods. (C) 2003 Elsevier Science B.V. All rights reserved.
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