期刊
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
卷 67, 期 2, 页码 87-96出版社
WILEY-LISS
DOI: 10.1002/arch.20223
关键词
Bombyx mori; EST; antibacterial protein; Gloverin
To identify genes involved in the innate immunity of the silkworm Bombyx mori, we constructed a cDNA library from the fat body of Escherichia coli-challenged B. mori larvae. Based on the expressed sequence tag (EST) data and whole genome shotgun sequence analysis, we found four Gloverin-like genes, BmGlov1-4, in the Bombyx genome. Northern blot and RTPCR analysis showed that BmGlov1-4 were induced in the larval fat body after an immune challenge by the injection of E. coli, however, less induction was observed after the injection of a yeast Candida albicons. In silico sequence analysis revealed the presence of a motif homologous to NF-kappa B binding site in the upstream region of each BmGlov gene. Moreover, we expressed recombinant BmGlov1-4 proteins using the baculovirus expression system, and found that all the recombinant BmGlov1-4 significantly inhibited the growth of E. coli.
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