期刊
FEMS MICROBIOLOGY LETTERS
卷 227, 期 2, 页码 197-202出版社
OXFORD UNIV PRESS
DOI: 10.1016/S0378-1097(03)00681-5
关键词
NiFe-hydrogenase; anaerobic respiration; hydrogenase maturation; Wolinella succinogenes
类别
Wolinella succinogenes grows by anaerobic respiration using hydrogen gas as electron donor. The hydE gene is located on the genome downstream of the structural genes encoding the membrane-bound NiFe-hydrogenase complex (HydABC) and a putative protease (HydD) possibly involved in hydrogenase maturation. Homologs of hydE are found in the vicinity of NiFe-hydrogenase-encoding genes on the genomes of several other proteobacteria. A hydE deletion mutant of W succinogenes does not catalyze hydrogen oxidation with various electron acceptors. The hydrogenase iron-sulfur subunit HydA is absent in mutant cells whereas the apparently processed NiFe subunit (HydB) is located exclusively in the soluble cell fraction. It is suggested that HydE is involved in the maturation and/or stability of HydA or the HydAB complex in some, but not all bacteria containing NiFe-hydrogenases. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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