期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1017, 期 1-2, 页码 151-159出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(03)01293-7
关键词
headspace analysis; stable isotope dilution; solid-phase microextraction; pectin; methanol; acetic acid; polysaccharides
A simple, fast, and direct procedure was developed for the simultaneous determination of the methanol and acetic acid present as esters in the plant cell wall polysaccharide pectin. After base-hydrolysis of esters and acidification of pectin samples, headspace solid-phase microextraction (SPME) was performed using a Carboxen-PDMS fiber assembly. Methanol and acetic acid were separated by gas chromatography with a Chrompak PoraPlot Q capillary column and detected using electron impact mass spectrometry with selected ion monitoring. Stable deuterated isotopomers (d(3)-methanol and d(3)-acetic acid) were used as internal standards and for constructing calibration curves, providing accurate and absolute quantification of analytes. The methanol and acetic acid contents in 1 mg quantities of fruit and vegetable pectins were readily quantified by this procedure. (C) 2003 Elsevier B.V. All rights reserved.
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