期刊
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
卷 51, 期 11, 页码 1567-1574出版社
HISTOCHEMICAL SOC INC
DOI: 10.1177/002215540305101116
关键词
intestine; sugar transport; fructose; GLUT5; SGLT1
类别
资金
- Wellcome Trust Funding Source: Medline
We have proposed a new model of intestinal sugar absorption in which high sugar concentrations promote rapid insertion of the facilitative transporter GLUT2 into the brush-border membrane so that absorptive capacity is precisely regulated to match dietary intake during the assimilation of a meal. However, location of GLUT2 at the brush border by immunocytochemistry has been problematical. We report that control of rapid GLUT2 trafficking and the use of an antibody to a sequence within the large extracellular loop of GLUT2 permits localization of GLUT2 at the brush border. To reveal brush-border GLUT2 fully, it is necessary to digest the sugar chain at the glycosylation site close to the antigenic site. In this way, we have demonstrated by immunocytochemistry PKC-dependent changes in the regulation of brush-border GLUT2 in rat jejunum that correspond to those seen by Western blotting. The functional and immunocytochemical data are now reconciled.
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