期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
卷 67A, 期 2, 页码 559-570出版社
WILEY
DOI: 10.1002/jbm.a.10120
关键词
bone marrow stromal cells; ligament tissue engineering; RGD; silk; biomaterial
资金
- NIAMS NIH HHS [R01 AR46563-01] Funding Source: Medline
- NIDCR NIH HHS [R01 DE 13405-01] Funding Source: Medline
Adhesion, spreading, proliferation, and collagen matrix production of human bone marrow stromal cells (BMSCs) on an RGD-modified silk matrix was studied. Anterior cruciate ligament fibroblasts (ACLFs) were used as a control cell source. Scanning electron microscopy (SEM) and MTT analyses demonstrated that the modified silk matrices support improved BMSC and ACLF attachment and show higher cell density over 14 days in culture when compared with the non-RGD-modified matrices. Collagen type I transcript levels (at day 7) and content (at day 14) was significantly higher on the RGD-modified substrate than on the nonmodified group. The ability of RGD-coupled silk matrices to support BMSC attachment, which leads to higher cell density and collagen matrix production in vitro, combined with mechanical, fatigue, and biocompatibility properties of the silk protein matrix, suggest potential for use of this biomaterial for tissue engineering. (C) 2003 Wiley Periodicals, Inc.
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