期刊
NATURE CELL BIOLOGY
卷 5, 期 11, 页码 967-971出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ncb1057
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- Biotechnology and Biological Sciences Research Council [BBS/E/J/00000129] Funding Source: researchfish
- Biotechnology and Biological Sciences Research Council [BBS/E/J/00000129] Funding Source: Medline
In plants, it is unclear how dispersed cortical microtubules are nucleated, polarized and organized in the absence of centrosomes. In Arabidopsis thaliana cells, expression of a fusion between the microtubule-end-binding protein AtEB1a and green fluorescent protein (GFP) results in labelling of spindle poles, where minus ends gather. During interphase, AtEB1a-GFP labels the microtubule plus end as a comet, but also marks the minus end as a site from which microtubules can grow and shrink. These minus-end nucleation sites are mobile, explaining how the cortical array can redistribute during the cell cycle and supporting the idea of a flexible centrosome in plants.
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