期刊
SYNAPSE
卷 50, 期 2, 页码 95-109出版社
WILEY-LISS
DOI: 10.1002/syn.10247
关键词
calcium channel; GABA(B) receptors; Kir3/GIRK channel; electrophysiology; two-photon confocal imaging
Gabapentin is a clinically effective anticonvulsant with an unclear mechanism of action. It was described as a GABA(B(1a,2)) receptor subtype-selective agonist, activating postsynaptic K+ currents and inhibiting postsynaptic Ca2+ channels in CA1 pyramidal cells, but without presynaptic actions. These activities appeared controversial and we therefore sought to further clarify gabapentin actions in rat hippocampal slices by characterizing K+ currents and Ca2+ channels targeted by gabapentin using whole-cell recording and multiphoton Ca2+ imaging. 1) We found that gabapentin and baclofen induced inwardly rectifying K+ currents (F-Gbp and K-Bac, respectively), sensitive to Ba2+ and Cs+. 2) A constitutively active K-IR current, independent of GABA(B) receptor activation and sensitive to Ba2+ and Cs+ was also present. 3) K-Gbp, K-Bac, and K-IR currents showed some differences in sensitivity to Ba2+ and Cs+, indicating the possible activation of distinct Kir3 currents, independent of KIR, by gabapentin and baclofen. 4) Gabapentin inhibition of Ca2+ channels was abolished by omega-conotoxin GVIA, but not by omega-agatoxin IVA and nimodipine, indicating a predominant action of gabapentin on N-type Ca2+ channels. 5) Gabapentin actions were linked to activation of pertussis toxin-sensitive G-proteins since N-ethylmaleimide (NEM) blocked K-Gbp activation and Ca2+ channel inhibition by gabapentin. 6) Finally, gabapentin reduced epileptiform discharges in slices via GABAB receptor activation. The anticonvulsant actions of gabapentin in hippocampal cells may thus involve GABAB receptor coupling to G-proteins and modulation of Kir3 and N-type Ca2+ channels. Moreover, gabapentin and baclofen activation of GABA(B) receptors may couple to distinct cellular targets. (C) 2003 Wiley-Liss, Inc.
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