期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 125, 期 44, 页码 13541-13547出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja035479k
关键词
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We report a real-time DNA detection method that utilizes single-strand DNA-modified nanoparticle probes and micropatterned chemoresponsive diffraction gratings interrogated simultaneously at multiple laser wavelengths. The surface-bound nanoparticle probe based assay with the chemoresponsive diffraction grating signal transduction scheme results in an experimentally simple DNA detection protocol, displaying attributes of both detection methodologies: the high sensitivity and selectivity afforded by nanoparticle probes and the experimental simplicity, wavelength-dependent resonant enhancement features, and miniaturization potential provided by the diffraction-based sensing technology.
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