期刊
JOURNAL OF CELL BIOLOGY
卷 163, 期 3, 页码 649-659出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200303134
关键词
selectin; rolling; lymph nodes; aggregation; shear flow
类别
资金
- NHLBI NIH HHS [R01 HL064831, 5-R24-HL64831] Funding Source: Medline
L-selectin is a key lectin essential for leukocyte capture and rolling on vessel walls. Functional adhesion of L-selectin requires a minimal threshold of hydrodynamic shear. Using high temporal resolution videomicroscopy, we now report that L-selectin engages its ligands through exceptionally labile adhesive bonds (tethers) even below this shear threshold. These tethers share a lifetime of 4 ms on distinct physiological ligands, two orders of magnitude shorter than the lifetime of the P-selectin-PSGL-1 bond. Below threshold shear, tether duration is not shortened by elevated shear stresses. However, above the shear threshold, selectin tethers undergo 1 4-fold stabilization by shear-driven leukocyte transport. Notably, the cytoplasmic tail of L-selectin contributes to this stabilization only above the shear threshold. These properties are not shared by P-selectin- or VLA-4-mediated tethers. L-selectin tethers appear adapted to undergo rapid avidity enhancement by cellular transport, a specialized mechanism not used by any other known adhesion receptor.
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