期刊
BLOOD
卷 102, 期 10, 页码 3478-3482出版社
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2003-05-1432
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- NCI NIH HHS [P01CA59318] Funding Source: Medline
- NHLBI NIH HHS [P01HL07104, P50HL54850] Funding Source: Medline
The standard approach to assess hematopoietic stem cell (HSC) engraftment in experimental bone marrow transplantation models relies on detection of donor hematopoietic cells in host bone marrow following death; this approach provides data from only a single time point after transplantation for each animal. In vivo bioluminescence imaging was therefore explored as a method to gain a dynamic, longitudinal profile of human HSC engraftment in a living xenogeneic model. Luciferase expression using a lentiviral vector allowed detection of distinctly different patterns of engraftment kinetics from human CD34(+) and CD34(+)CD38(-) populations in the marrow NOD/SCID/beta2m(null) mice. Imaging showed an early peak (day 13) of engraftment from CD34(+) cells followed by a rapid decline in signal. Engraftment from the more primitive CD34(+)CD38(-) population was relatively delayed but by day 36 increased to significantly higher levels than those from CD34(+) cells (P < .05). Signal intensity from CD34(+)CD38(-)-engrafted mice continued to increase during more than 100 days of analysis. Flow cytometry analysis of bone marrow from mice after death demonstrated that levels of 1% donor cell engraftment could be readily detected by bioluminescence imaging; higher engraftment levels corresponded to higher image signal intensity. In vivo bioluminescence imaging provides a novel method to track the dynamics of engraftment of human HSC and progenitors in vivo. (C) 2003 by The American Society of Hematology.
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