期刊
NUCLEIC ACIDS RESEARCH
卷 31, 期 22, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gng140
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资金
- NCI NIH HHS [P01 CA92625-0, P01 CA092625] Funding Source: Medline
- NIAID NIH HHS [R37 AI054636] Funding Source: Medline
The Cre/loxP recombination system is a commonly used tool to alter the mouse genome in a conditional manner by deletion or inversion of loxP-flanked DNA segments. While Cre-mediated deletion is essentially unidirectional, inversion is reversible and therefore does not allow the stable alteration of gene function in cells that constitutively express Cre. Site-directed mutagenesis yielded a pair of asymmetric loxP sites (lox66 and lox71) that display a favorable forward reaction equilibrium. Here, we demonstrate that lox66/lox71 mediates efficient and predominantly unidirectional inversion of a switch substrate targeted to the mouse genome in combination with either inducible or cell type-specific cre-transgenes in vivo.
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