期刊
NEUROCHEMICAL RESEARCH
卷 28, 期 12, 页码 1873-1881出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1023/A:1026179926780
关键词
cDNA microarray; Parkinson's disease; 1-methyl-4-phenyl-pyridinium; gene expression; neurodegeneration
资金
- NIA NIH HHS [P30AG13846, R37AG05894] Funding Source: Medline
cDNA microarray analysis of 1-methyl-4-phenyl-pyridinium (MPP+) toxicity (1 mM, 72 h) in undifferentiated SH-SY5Y cells identified 48 genes that displayed a signal intensity greater than the mean of all differentially expressed genes and a two-fold or greater difference in normalized expression. RT-PCR analysis of a subset of genes showed that c-Myc and RNA-binding protein 3 (RMB3) expression decreased by similar to50% after 72 h of exposure to MPP+ (1 mM) but did not change after 72 h of exposure to 6-hydroxydopamine (25 muM), rotenone (50 nM), and hydrogen peroxide (600 muM). Exposure of retinoic acid (RA)-differentiated SH-SY5Y cells to MPP+ (1 mM, 72 h) also resulted in a decrease in RMB3 expression and an increase in GADD153 expression. In contrast, c-Myc expression was slightly increased in RA-differentiated cells. Collectively, these data provide new insights into the molecular mechanisms of MPP+ toxicity and show that MPP+ can elicit distinct patterns of gene expression in undifferentiated and RA-differentiated SH-SY5Y cells.
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