期刊
INSECT MOLECULAR BIOLOGY
卷 12, 期 6, 页码 651-658出版社
BLACKWELL PUBLISHING LTD
DOI: 10.1046/j.1365-2583.2003.00451.x
关键词
baculovirus; late expression factors; replication; host specificity
Autographa californica multiple nucleopolyhedrovirus (AcMNPV) infects a wide variety of insect species. A number of AcMNPV late expression factors that are involved in replication have been identified as playing a role in determining host specificity. Host cell factor-1, or HCF-1, was previously demonstrated to be essential for viral replication in Tn-368 cells. Here we demonstrate that HCF-1 is an early protein and localizes to the cell nucleus. Coprecipitation experiments revealed that HCF-1 interacts with itself but none of the other late expression factors required for replication in Tn-368 cells. HCF-1 mutants were constructed and utilized to search for the domains involved in HCF-1 biological function and oligomerization. Possible roles of HCF-1 in determining host specificity are discussed.
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