期刊
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
卷 285, 期 6, 页码 G1162-G1170出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpgi.00243.2003
关键词
neonates; permeability; tight junctions; intraepithelial lymphocytes; goblet cells
资金
- NICHD NIH HHS [T32-HD-07445, HD-33920] Funding Source: Medline
Sepsis is the most common morbidity in preterm infants, who often receive total parenteral nutrition (TPN). We hypothesized that gut barrier function is compromised in TPN-fed compared with enterally fed newborn piglets (ENT pigs). Colostrum-deprived newborn pigs were implanted with jugular venous and bladder catheters under general anesthesia. Pigs were either administered TPN (n = 15) or fed formula (ENT pigs, n = 15). After 6 days, pigs were gavaged a solution of mannitol, lactulose, and polyethylene glycol 4000 ( PEG 4000) and urine was collected for 24 h. At 7 days, small bowel samples were assayed for myeloperoxidase activity, morphometry, and tight junction protein abundance. Intestinal contents and peripheral organ sites were cultured for bacteria. Urinary recovery (% dose) of mannitol ( 53 vs. 68) was lower, whereas that of lactulose ( 2.93 vs. 0.18) and PEG 4000 ( 12.78 vs. 0.96) were higher in TPN vs. ENT pigs, respectively ( P < 0.05). Incidence of translocation was similar in TPN and ENT pigs. Myeloperoxidase activity was increased in TPN vs. ENT pigs in the jejunum ( P < 0.001) and was weakly correlated with lactulose (R-2 = 0.32) and PEG 4000 (R-2 = 0.38) recovery. Goblet cell counts did not change, but intraepithelial lymphocyte numbers decreased with TPN. Only claudin-1 protein abundance was increased in the TPN group. We conclude that TPN is associated with impairment of neonatal gut barrier function as measured by permeability but not translocation.
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