期刊
MOLECULAR AND CELLULAR BIOLOGY
卷 23, 期 24, 页码 9283-9292出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.23.24.9283-9292.2003
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资金
- NIA NIH HHS [R01 AG018000, AG 18000] Funding Source: Medline
- NIGMS NIH HHS [R37 GM026020, GM 26020, R01 GM026020] Funding Source: Medline
We have identified a novel tRNA methyltransferase in Saccharomyces cerevisiae that we designate Trm9. This enzyme, the product of the YML014w gene, catalyzes the esterification of modified uridine nucleotides, resulting in the formation of 5-methylcarbonylmethyluridine in tRNA(Arg3) and 5-methylcarbonylmethyl-2-thiouridine in tRNA(Glu). In intact yeast cells, disruption of the TRM9 gene results in the complete loss of these modified wobble bases and increased sensitivity at 37degreesC to paromomycin, a translational inhibitor. These results suggest a role for this potentially reversible methyl esterification reaction when cells are under stress.
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