Glucoarnylase, α-amylase and β-amylase immobilisation on acrylic carriers

In this paper immobilisation effectivity of alpha-amylase, beta-amylase and glucoamylase on copolymers of butyl acrylate and pentaerhitrite triacrylate or ethylene glycol dimethacrylate was evaluated. Special attention was focused on copolymers having three kinds of anchor groups: -OH, -COOH, and -NH2. The anchor groups were generated on polymer matrices by aminolysis with ethylenediamine. Particular carrier with the same structure was activated by glutaraldehyde, divinyl sulfone or carbodiimide procedure. The first method was found to give enzyme-carrier preparations of the highest activities. From seven investigated carriers the copolymer of butyl acrylate and ethylene glycol dimethacrylate was found as the best for three amylolytic enzymes immobilisation. The activities of selected immobilised enzyme preparations and thermal and pH stability were determined. These efforts resulted in selection of preparation based on one polymer matrix to form dual enzyme systems: alpha-amylase and beta-amylase, or alpha-amylase and glucoamylase. (C) 2003 Elsevier Science B.V. All rights reserved.