期刊
DNA REPAIR
卷 2, 期 12, 页码 1419-1427出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.dnarep.2003.08.012
关键词
deinoccocus radiodurans; radiation; DNA damage and repair
资金
- NCI NIH HHS [R01CA073764] Funding Source: Medline
PprI, a newly identified gene switch responsible for extreme radioresistance of Deinococcus radiodurans, plays a central regulatory role in multiple DNA damage repair and protection pathways in response to radiation stress [Biochem. Biophy. Res. Commun. 306 (2003) 354]. To evaluate whether PprI also functions in the radioresistance in other organisms, D. radiodurans PprI protein (Deira-PprI) was expressed in Escherichia coli. The complemented E. coli strain showed an increase of approximately 1.6-fold radioresistance with a high dose of gamma irradiation. Immunoblotting assays showed that the expression of Deira-PprI in E. coli resulted in a significant increase in RecA protein expression following high dose ionizing radiation. The expression of Deira-PprI protein also significantly enhanced the scavenging ability of free radicals by inducing the enzymatic activity of KatG. These results indicate that exogenous expression of Deira-PprI promotes DNA repair and protection pathways and enhances the radioresistance of E. coli. (C) 2003 Elsevier B.V. All rights reserved.
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