期刊
FEMS MICROBIOLOGY LETTERS
卷 229, 期 2, 页码 173-178出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0378-1097(03)00821-8
关键词
photosynthetic denitrifier; denitrification; nitrite reductase; nitric oxide reductase; regulation
类别
NnrR, a transcriptional activator and member of the CRP/FNR family of regulators, is responsible for controlling the expression of a number of denitrification genes in Rhodobacter sphaeroides 2.4.3. The apparent effector for NnrR is nitric oxide, and in its presence NnrR activates expression of the nirK gene and the nor operon, encoding nitrite reductase and nitric oxide reductase, respectively. Whether nitric oxide directly interacts with NnrR to activate transcription is unknown. Other denitrifiers carry putative orthologs of NnrR. To gain insight into NnrR function, a number of conserved residues were mutagenized. The impact of these changes on NnrR function was assessed by monitoring expression of a nirK-lacZ fusion. In this way a region spanning from Tyr93 to Cys103 that contains residues critical for NnrR activity was identified. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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