期刊
ANALYTICAL CHEMISTRY
卷 75, 期 24, 页码 6963-6967出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac0348524
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资金
- NHGRI NIH HHS [HG01506] Funding Source: Medline
In this paper, we report a factor of 3 improvement in the resolution and a factor of 10 improvement in the speed of fractionation of similar to100-kb DNA molecules in Brownian ratchet arrays. In our device, the electrophoretic flow is tilted at a small angle relative to the array axis. Tilting accelerates the fractionation speed because a higher fraction of the diffusing molecules is ratcheted at each step in the array. Molecules of lengths 48.5 and 164 kb can be separated in similar to70 min with a resolution of similar to3.8, using a 12-mm-long array. The Brownian ratchet arrays are not limited to DNA separation, but can, in principle, be used for any particle in this size range.
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