Combining deconvolution and fluctuation analysis to determine quantal parameters and release rates

Analysis methods are described which integrate information from fluctuation analysis with that from deconvolution. Together the two approaches allow to derive a consistent quantitative description of quantal release (both evoked, spontaneous and asynchronous) under conditions in which quantal parameters may change during a repetitively applied stimulation protocol. Specifically, our methods take into account the effects of accumulating transmitter in the synaptic cleft and postsynaptic receptor desensitization, which may develop during strong stimulation. Several ways to handle non-stationatities are described. Examples are provided for the Calyx of Held, a glutamatergic synapse, in which both the pre- and the postsynaptic compartments can be voltage-clamped. (C) 2003 Elsevier B.V. All rights reserved.