期刊
BLOOD
卷 102, 期 13, 页码 4354-4360出版社
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2003-04-1308
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- NCI NIH HHS [CA-68485] Funding Source: Medline
- NIDDK NIH HHS [DK-15555] Funding Source: Medline
Polycythemia vera (PV) is a human clonal hematologic disorder. Previously we demonstrated that erythroid colony-forming cells (ECFCs) from PV patients contained a hyperactive membrane-associated tyrosine phosphatase. We now show that this phosphatase corresponded to protein tyrosine phosphatase (PTP)-MEG2, an intracellular enzyme with a putative lipid-binding domain. The increased activity of PTP-MEG2 in PV cells is due to its elevated distribution in the membrane fraction. With the development of ECFCs to mature red cells, the protein level of PTP-MEG2 decreased gradually, but membrane-associated PTP-MEG2 was sustained for a longer period of time in PV cells, which correlated with an enhanced colony-forming capability of the cells. Importantly, expression of dominant-negative mutant forms of PTP-MEG2 suppressed in vitro growth and expansion of both normal and PV ECFCs. The data indicate that PTP-MEG2 has an important role in the development of erythrold cells. (C) 2003 by The American Society of Hematology.
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