期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 798, 期 2, 页码 249-255出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2003.09.056
关键词
derivatization, GC; amphetamine; methamphetamine
A procedure based on gas chromatography-mass spectrometry (GC-MS) is described for the determination of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA, ecstasy), 3,4-methylenedioxyethylamphetamine (MDE or MDEA) and N-methyl-1-(3,4-methylenedioxyphenyl)-2-butanamine (MBDB) in hair. Hair samples were digested with 1 M sodium sulfide at 37 degreesC (by shaking for 3 h and was kept at room temperature overnight), and extracted with two sequential extraction procedures: liquid-liquid extraction with tert-butyl methyl ether and solid-phase extraction with Bond-Elut Certify columns. Extracted analytes were derivatised with N-methyl-bis(trifluoroacetamide), separated by a 5% phenylmethylsilicone column and determined by a mass spectrometer detector in selected ion monitoring mode. A good reproducibility (intra-assay R.S.D. = 1.5-15.7%), accuracy (intra-assay error = 2.0-11.7%) and sensitivity (LOD = 0.03-0.08 ng/mg hair) were attained. The method was successfully applied to the analysis of the proximal (I cm) hair segment to assess recent self-reported use in ecstasy consumers. Otherwise, further studies are needed to validate methodology developed in case of amphetamine consumption. (C) 2003 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据