期刊
JOURNAL OF MOLECULAR BIOLOGY
卷 335, 期 1, 页码 307-319出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2003.10.030
关键词
allosteric effector; autoinhibition; protein fold; restriction endonuclease; type IIE endonuclease
Eco RII is a type IIE restriction endonuclease that interacts with two copies of the DNA recognition sequence 5'CCWGG, one being the actual target of cleavage, the other serving as the allosteric effector. The mode of enzyme activation by effector binding is unknown. To investigate the molecular basis of activation and cleavage mechanisms by Eco RII, the crystal structure of Eco RII mutant R88A has been solved at 2.1 Angstrom resolution. The Eco RII monomer has two domains linked through a hinge loop. The N-terminal effector-binding domain has a novel DNA recognition fold with a prominent cleft. The C-terminal catalytic domain has a restriction endonuclease-like fold. Structure-based sequence alignment identified the putative catalytic site of Eco RII that is spatially blocked by the N-terminal domain. The structure together with the earlier characterized Eco RII enzyme activity enhancement in the absence of its N-terminal domain reveal an autoinhibition/activation mechanism of enzyme activity mediated by a novel effector-binding fold. This is the first case of autoinhibition, a mechanism described for many transcription factors and signal transducing proteins, of a restriction endonuclease. (C) 2003 Elsevier Ltd. All rights reserved.
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