期刊
FEBS LETTERS
卷 556, 期 1-3, 页码 64-68出版社
WILEY
DOI: 10.1016/S0014-5793(03)01369-3
关键词
NADH : ubiquinone oxidoreductase; complex I; active/de-active transition; succinate dehydrogenase; complex II; Krebs cycle; regulation; oxygen; mitochondrion
资金
- NHLBI NIH HHS [HL68738] Funding Source: Medline
- NIGMS NIH HHS [GM61606] Funding Source: Medline
Two main entry points for electrons into the mitochondrial respiratory chain are NADH:ubiquinone oxidoreductase (complex I) and succinate:ubiquinone oxidoreductase (complex II). Metabolic regulation of these two respiratory complexes is not understood in detail. It has been suggested that the Krebs cycle metabolic intermediate oxaloacetate (OAA) inhibits complex II in vivo, whereas complex I undergoes a reversible active/de-active transition. In normoxic and anoxic hearts it has been shown that the proportion of complex I in the active and de-active states is different suggesting a possible mode of regulation of the enzyme by oxygen concentration. In the current studies rapid isolation of mitochondrial membranes in a state that preserves the activity of both complex I and complex II has been achieved using Langendorff perfused rat hearts. The findings indicate that the state of activation of complex I is controlled by the oxygen saturation in the perfusate. In addition, these studies show that complex II is fully active in the mitochondrion and not inhibited by OAA regardless of the oxygen concentration. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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