The 2.0 A resolution crystal structure of prostaglandin H2 synthase-1:: Structural insights into an unusual peroxidase

Prostaglandin H-2 synthase (EC 1.14.99.1) is an integral membrane enzyme containing a cyclooxygenase site, which is the target for the non-steroidal anti-inflammatory drugs, and a spatially distinct peroxidase site. Previous crystallographic studies of this clinically important drug target have been hindered by low resolution. We present here the 2.0 Angstrom resolution X-ray crystal structure of ovine prostaglandin H-2 synthase-1 in complex with alpha-methyl-4-biphenylacetic-methyl-4-biphenylacetic acid, a defluorinated analog of the non- steroidal anti-inflammatory drug flurbiprofen. Detergent molecules are seen to bind to the proteins membrane-binding domain, and their positions suggest the depth to which this domain is likely to penetrate into the lipid bilayer. The relation of the enzyme's proximal heme ligand His388 to the heme iron is atypical for a peroxidase; the iron-histidine bond is unusually long and a substantial tilt angle is observed between the heme and imidazole planes. A molecule of glycerol, used as a cryoprotectant during diffraction experiments, is seen to bind in the peroxidase site, offering the first view of any ligand in this active site. Insights gained from glycerol binding may prove useful in the design of a peroxidase-specific ligand. (C) 2003 Elsevier Ltd. All rights reserved.