期刊
FEBS LETTERS
卷 557, 期 1-3, 页码 265-268出版社
WILEY
DOI: 10.1016/S0014-5793(03)01494-7
关键词
mitochondrial small beat-shock protein; thermotolerance; tobacco; transformation
To clarify the role of mitochondrial small heat-shock protein (MT-sHSP) in the heat-shock response, we introduced the tomato (Lycopersicon esculentum) MT-sHSP gene under the control of the 35S promoter into tobacco (Nicotiana tabacum), and examined the thermotolerance of the transformed plants. Irrespective of the orientation, sense or antisense, of the gene, the transgenic plants exhibited a normal morphology and growth rate in the vegetative growth stage. When 4-week-old seedlings were exposed to sudden heat stress, the sense plants which overexpress the MT-sHSP gene exhibited thermotolerance, whereas the antisense plants in which the expression of the gene is suppressed exhibited susceptibility. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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