期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1023, 期 2, 页码 317-320出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2003.10.029
关键词
sample preparation; proteomics; proteins
Protein samples should be free of salt and other disturbing agents and have an appropriate concentration to be suitable for two-dimensional (2D) electrophoresis, the principal step of proteomics. To find the most efficient method for sample preparation, we used human plasma and compared four widely applied precipitation methods, using trichloroacetic acid (TCA), acetone, chloroform/methanol and ammonium sulfate, as well as ultrafiltration. Precipitation with TCA and acetone and ultrafiltration resulted in an efficient sample concentration and desalting. We also found that ammonium sulfate fractionation can efficiently remove albumin, which represents more than 50% of plasma proteins. (C) 2003 Elsevier B.V. All rights reserved.
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