期刊
JOURNAL OF CELL BIOLOGY
卷 164, 期 2, 页码 255-266出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200308132
关键词
cilia; intraflagellar; transport; kinesin; radial spoke
类别
资金
- NIGMS NIH HHS [GM14642, R01 GM014642, R37 GM014642] Funding Source: Medline
Intraflagellar transport (IFT) is the bidirectional movement of multisubunit protein particles along axonemal microtubules and is required for assembly and maintenance of eukaryotic flagella and cilia. One posited role of IFT is to transport flagellar precursors to the flagellar tip for assembly. Here, we examine radial spokes, axonemal subunits consisting of 22 polypeptides, as potential cargo for IFT. Radial spokes were found to be partially assembled in the cell body, before being transported to the flagellar tip by anterograde IFT. Fully assembled radial spokes, detached from axonemal microtubules during flagellar breakdown or turnover, are removed from flagella by retrograde IFT. Interactions between IFT particles, motors, radial spokes, and other axonemal proteins were verified by coimmuno-precipitation of these proteins from the soluble fraction of Chlamydomonas flagella. These studies indicate that one of the main roles of IFT in flagellar assembly and maintenance is, to transport axonemal proteins in and out of the flagellum.
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