期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 63, 期 35, 页码 7862-7872出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.5b02697
关键词
trichothecenes; masked mycotoxins; cereals; liquid chromatography-high resolution mass spectrometry; metabolomics; stable isotopes
资金
- Austrian Federal Ministry of Science, Research and Economy
- National Foundation for Research, Technology and Development of Austria
- BIOMIN Holding GmbH
- Austrian Science Fund (FWF) [P26213-B22, SFB F3706]
- Finnish Funding Agency for Technology and Innovation (TEKES
- Myco-DETECT Project) [401/31/2011]
- Walter Ehrstrom Foundation
- Vienna Science and Technology Fund project [WWTF LS12-012]
- FWF project [SFB F3701]
- Austrian Science Fund (FWF) [P26213] Funding Source: Austrian Science Fund (FWF)
- Austrian Science Fund (FWF) [P 26213] Funding Source: researchfish
To investigate the metabolic fate of HT-2 toxin (HT2) and T-2 toxin (T2) in wheat (Triticum aestivum L.), an untargeted metabolomics study utilizing stable isotopic labeling and liquid chromatography high resolution mass spectrometry was performed. In total, 11 HT2 and 12 T2 derived in planta biotransformation products were annotated putatively. In addition to previously reported mono- and diglucosylated forms of HT2, evidence for the formation of HT2-malonyl-glucoside and feruloyl-T2, as well as acetylation and deacetylation products in wheat was obtained for the first time. To monitor the kinetics of metabolite formation, a time course experiment was conducted involving the Fusarium head blight susceptible variety Remus and the resistant cultivar CM-82036. Biotransformation reactions were observed already at the earliest tested time point (6 h after treatment), and formed metabolites showed different kinetic profiles. After ripening, less than 15% of the toxins added to the plants were determined to be unmetabolized.
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