期刊
EMBO JOURNAL
卷 23, 期 2, 页码 294-301出版社
WILEY
DOI: 10.1038/sj.emboj.7600063
关键词
membrane insertion; reconstitution; Sec-independence; translocation; YidC
资金
- NIGMS NIH HHS [GM63862, R01 GM063862] Funding Source: Medline
YidC is a recently discovered bacterial membrane protein that is related to the mitochondrial Oxa1p and the Alb3 protein of chloroplasts. These proteins are required in the membrane integration process of newly synthesized proteins that do not require the classical Sec machinery. Here we demonstrate that YidC is sufficient for the membrane integration of a Sec-independent protein. Microgram amounts of the purified single-spanning Pf3 coat protein were efficiently inserted into proteoliposomes containing the purified YidC. A mutant Pf3 coat protein with an extended hydrophobic region was inserted independently of YidC into the membrane both in vivo and in vitro, but its insertion was accelerated by YidC. These results show that YidC can function separately from the Sec translocase to integrate membrane proteins into the lipid bilayer.
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