期刊
DEVELOPMENTAL BRAIN RESEARCH
卷 148, 期 1, 页码 77-87出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.devbrainres.2003.10.014
关键词
beta-AR; P2Y; multiplex single cell RT-PCR; Ca2+ imaging; freshly isolated astrocyte; rat hippocampus
资金
- NINDS NIH HHS [NS19492] Funding Source: Medline
Although almost all GFAP(+) cells in primary astrocyte cultures show functional beta-adrenergic (beta-AR) and metabotropic purinergic (P2Y) receptors, the fewer studies on astrocytes in situ have shown that a much smaller proportion express these same receptor-mediated activities. Here we show, by multiplex single cell RT-PCR, that 44% of freshly isolated, GFAP(+) astrocytes (FIAs) from the CA1 of P8-12 rat hippocampus always co-express beta-adrenergic receptor mRNA subtypes with metabotropic ATP receptor mRNA subtypes (P2Y1, P2Y2 or P2Y4). We also found that beta2 mRNA was the dominant beta-AR subtype expressed. P2Y I mRNA always co-expresses with either one or two subtypes of P2U-Iike receptor (P2Y2 or P2Y4) mRNAs. Immunocytochemical studies showed a similar percentage of all FIAs expressed beta-AR and P2Y1 protein (54% and 52%, respectively), as for the mRNAs (46% and 65%, respectively). The staining of hippocampal sections for beta-AR or P2Y I receptor plus GFAP shows that there are quite numerous, scattered star-shaped GFAP(+) astrocytes in the CAI region of P9-10 rat hippocampus that stained positive for either of these receptors. These data show that astrocytes in situ express, and to a large extent likely co-express, beta-AR and P2Y receptors. (C) 2003 Elsevier B.V. All rights reserved.
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