期刊
GENES & DEVELOPMENT
卷 18, 期 3, 页码 344-354出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.1167904
关键词
DNA looping; protein-protein interactions; multimerization; transcriptional control; lysogeny
资金
- NIGMS NIH HHS [R01 GM062976, R01 GM062976-03] Funding Source: Medline
Effective repression of cI transcription from P-RM by the bacteriophage gimel CI repressor requires binding sites (O-L) located 2.4 kb from the promoter. A CI tetramer bound to O(L)1.O(L)2 interacts with a tetramer bound near PRM (O(R)1.O(R)2), looping the intervening DNA. We previously proposed that in this CI octamer:DNA complex, the distant O(L)3 operator and the weak O(R)3 operator overlapping P-RM are juxtaposed so that a CI dimer at O(L)3 can cooperate with a CI dimer binding to O(R)3. Here we show that O(L)3 is necessary for effective repression of P-RM and that the repressor at O(L)3 appears to interact specifically with the repressor at O(R)3. The O(L)3-CI-O(R)3 interaction involves the same CI interface used for short-range dimer-dimer interactions and does not occur without the other four operators. The long-range interactions were incorporated into a physicochemical model, allowing estimation of the long-range interaction energies and showing the lysogenic state to be ideally poised for CI negative autoregulation. The results establish the X system as a powerful tool for examining long-range gene regulatory interactions in vivo.
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