期刊
INSECT MOLECULAR BIOLOGY
卷 13, 期 1, 页码 89-100出版社
BLACKWELL PUBLISHING LTD
DOI: 10.1111/j.1365-2583.2004.00464.x
关键词
Sindbis; alphavirus; mosquito; Bombyx; Manduca; viral dissemination
资金
- FIC NIH HHS [R03TWO1209-01] Funding Source: Medline
- NIAID NIH HHS [R01 AI046435-02, R01 AI046435-03, AI46753, R01 AI046435-04A1, R01 AI046435, AI46435] Funding Source: Medline
Alphavirus transducing systems (ATSs) are alphavirus-based tools for expressing genes in insects. Here we describe an ATS (5'dsMRE16ic) based entirely on Sindbis MRE16 virus. GFP expression was used to characterize alimentary tract infections and dissemination in three Culicine and two Lepidopteran species. Following per os infection, 5'dsMRE16ic-EGFP efficiently infected Aedes aegypti and Culex tritaeniorhynchus, but not Culex pipiens pipiens. Ae. aegypti clearly showed accumulation of green fluorescent protein (GFP) in the posterior midgut and foregut/midgut junction within 2-3 days postinfection. Following parenteral infection of larvae, Bombyx mori had extensive GFP expression in larvae and adults, but Manduca sexta larvae were mostly resistant. 5'dsMRE16ic should be a valuable tool for gene expression in several important insect species that are otherwise difficult to manipulate genetically.
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