期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 63, 期 6, 页码 1698-1710出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf505515n
关键词
free sugars; greezing effect; growth modulator; Medicago truncatula; multicomponent relaxation alalysis; NMR relaxometry; oligosaccharides; seed germination; water dynamics
资金
- Region Pays de la Loire Research Contract QUALISEM
The water status of Medicago truncatula Gaertn. seed was followed by low-field NMR relaxometry during germination with and without oryzalin or fusicoccin used as growth modulators. T-1 and T-2 relaxation times and proportions P-1 and P-2 were determined on fresh, frozen, and freezethawed samples to characterize changes in water dynamics and compartmentation and in the nonfreezing water fraction. The results demonstrate that low-field NMR relaxometry allowed differentiating germination phases and events occurring during them as well as perturbations related to the presence of growth modulators. The results provide clear evidence that the classical multicomponent relaxation interpretation cannot directly relate T-2 components and morphological compartments in biological tissue.
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