期刊
BIOMATERIALS
卷 25, 期 4, 页码 617-624出版社
ELSEVIER SCI LTD
DOI: 10.1016/S0142-9612(03)00570-2
关键词
silk; fibronectin; RGD peptide; genetic engineering; cell adhesion; cell culture
There are a variety of silkworms and silk fibroins produced by them. Silks have many inherent suitable properties for biomaterials. In this paper, a novel silk-like hybrid protein, [DGG(A)(12)GGAASTGRGDSPAAS](5), which consists of polyalanine region of silk fibroin from a wild silkworm, Samia cynthia ricini, and cell adhesive region including Arg-Gly-Asp (RGD) sequence, derived from fibronectin, was designed and produced. The genes encoding the hybrid protein were constructed and expressed in Escherichia coli. The main conformation of the polyalanine region, that is, either alpha-helix or beta-sheet, could be easily controlled by treatment with different acidic solvents, trifluoroacetic acid or formic acid, respectively. This structural change was monitored with C-13 CP/MAS NMR. Higher cell adhesive and growth activities of the hybrid protein compared with those of collagen were obtained. (C) 2003 Elsevier Ltd. All rights reserved.
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