期刊
MOLECULAR BIOTECHNOLOGY
卷 26, 期 2, 页码 95-99出版社
HUMANA PRESS INC
DOI: 10.1385/MB:26:2:95
关键词
apple; grape; hot borate; RNA contamination; RNA extraction
An efficient, simple, and small-scale procedure for isolating functional ribonucleic acid (RNA) was successfully applied to many different tissues of grape and apple. These woody plants are rich in polyphenolic compounds and polysaccharides that could impair the RNA extraction. The:method chosen is based on the use of hot borate buffer at alkaline pH supplemented with-several adjuvants and followed by selective precipitations. Starting with only,0.4,g of fresh tissue and working with small tubes (2 mL), we were able to obtain good yields of high-quality RNA suitable for further applications. The procedure can be proposed for many applications, and it is particularly highly recommended when isolating RNA from a large number of samples.
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